Good Antibody for Identifying Macrophages

November 04, 2015

Microbiology
Medical College of Wisconsin
Research Technologist

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Company:

BioLegend

Product Name:

CD11b FITC

Catalog Number:

101205

Info: View Product View Product Specs

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Latently infected mouse PEC's were collected and stained, and CD11b+ cells were sorted out as macrophages from other immune cells.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Peritoneal Exudate Cells

Primary Incubation

1:100 15 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:500 15 minutes

Tertiary Incubation

NA

Detection

Facs Aria

Results Summary

This antibody identifies macrophage populations in latently infected cell populations.

Additional Notes

None

Related Categories

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Summary

The Good

Robust Stain

The Bad

Not always specific for just macrophages

The Bottom Line

Good, relatively inexpensive antibody to sort out macrophages from other immune cells in infected mouse cell populations.

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