PE Anti-Mouse CD4 Antibody

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

100407

Catalog Number:

PE Anti-mouse CD4 Antibody

The purpose of this study was to identify the population of CD4 positive lymphocytes from mediastinal lymph nodes that express certain cytokines. This antibody was used in combination with antibodies for cytokines of interest. The product was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mediastinal lymph node cells

Primary Incubation

1:100, in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

Single cell suspensions of mediastinal lymph node cells were stained with the antibody as described. The antibody allowed for clear identification of CD4 positive cells, which were then successfully analyzed for their production of certain cytokines (i.e. interleukins etc).

Additional Notes

None

Related Categories

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Summary

The Good

Clean antibody

The Bad

None

The Bottom Line

Very good antibody.

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