BD LSR Fortessa X-20 Cell Analyzer

December 10, 2015

BlueskyReddit
Rigel Pharmaceutical
Immunology
Associate Scientist

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Company:

Rigel

Product Name:

BD LSR Fortessa X-20 Cell Analyzer

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The BD LSR Fortessa X-20 Cell Analyzer is a great instrument for looking at multiple cell markers within a sample. Only one sample is needed for up to 18 colors. The ability to look at multiple markers allows for better profiling of samples. This is especially useful for in vivo studies. In our studies, we were able to look at T cells, B cells, Monocytes, Macrophages, Neutrophils, and Dendritic cells, all within one sample.

Experimental Design and Results Summary

Tips

Compensation can be done on the Diva software the Fortessa is linked to or compensation can be done on Flow Jo if that is the software analysis that is being used.

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Summary

The Good

Quick and efficient results

The Bad

This instrument does require training and takes a while to set up before you can run your samples.

The Bottom Line

The BD LSR Fortessa X-20 is a very useful instrument to have in the lab. It has a lot of quirks and takes a few tries for new users to be comfortable using it. However, with the ability to detect multi-color parameters all within one sample, a lot of time is saved during setup. Users can better profile their samples quickly and efficiently. This instrument is highly recommended.

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