Merck Millipore
Anti-mTOR, clone F11
MABS14
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). Here, we performed Western blot analyses of mTOR protein expression in cell lysates of human Podocytes using the Anti-mTOR, clone F11 antibody.
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% Skim milk in TBST buffer
Dilution: 1:5000, 1 hour, 24°C
ECL - 5 min, room temperature
ECL
A band including mTOR (289 kDa) was detected after 5 min
None
A band including mTOR (289 kDa) was detected
This antibody is well suited for Western blot analyses of mTOR protein expression in the cell lysate of human podocytes