Flowmi for MIJ Preps

The Jackson Laboratory
Genetic Engineering Technologies
ES Cell Technologist

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
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Company:

Bel-Art Products

Product Name:

Scienceware Flowmi Cell Strainer

Catalog Number:

H13680-0040

I tested this product to see if it would assist with isolating the ES cells from the MEF cells in our culture preps for Microinjection. The product worked excellently and produced a cleaner prep over our conventional operations.

Experimental Design and Results Summary

Application

Microinjection

Starting Material

ES cell culture on feeders

Protocol Overview

Trypsinize cell colonies to achieve single-cell suspension, deactivate with media, and strain through Flowmi filter to pass ES cells and retain MEF cells.

Tips

None

Results Summary

We achieved a cleaner prep with a higher ES cell yield.

Features Summary

Very easy to use

Additional Notes

Did not observe any leaking from tip insertion point. All culture went through the filter.

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Summary

The Good

Ease of use.

The Bad

Limited to pore sizes of 40um and 70um.

The Bottom Line

I would highly recommend this product.

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