Sigma-Aldrich
Monoclonal Anti-Brg1, Clone 5B7
SAB4200195
Browse Similar Products: Brg1 Immunofluorescence Antibodies
Our interest is in coregulators in mouse and human pancreas, specifically in islet alpha and beta cells. We are focused on evaluating the Swi/Snf complex in islet cell functions. We utilized the Anti-Brg1 (Rat) antibody from Sigma to evaluate the distribution of this essential ATPase sub-unit throughout the pancreas, and more specifically, the islet.
Immunofluorescence
OCT-embedded mouse pancreas
1:500; 4 deg C; Overnight
5% Normal Donkey Serum in 1%BSA/PBS (w/v)
1:1000. Rat IgG-Cy3. 2 hours, Room temperature
NA
Fluorescence microscopy
The Brg1 antibody worked great throughout the entire pancreas section. It is ubiquitously expressed in all pancreas cell types (e.g. endocrine and exocrine). Insulin and Glucagon primary antibodies were used to detect islet beta and alpha cells, respectively.
None
Good detection, low background at 1:500
This antibody worked great for immunofluorescence in cryosections. We will be using again once our stock runs out.