Good Machine for Four Color Staining

University of California
Rheumatology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD FACS Calibur

Catalog Number:

BD FACSCalibur

FACS calibur was used to run the stained cells from mouse lymph nodes.

Experimental Design and Results Summary

Tips

PE- Cy5 leaks extensively in FL2 and FL3 unless voltages are adjusted properly and dilution maintained

Image Gallery

Summary

The Good

Easy to use and clean up

The Bad

Clogs fast and slow to run

The Bottom Line

Overall good if you want to do a quick two or three color staining procedure

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