p-FoxO3 (S253) Antibody from Novus Biologicals

December 10, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

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Quality of Results

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Company:

Novus Biologicals

Product Name:

p-FoxO3 (S253) Antibody

Catalog Number:

NBP1-19927

This antibody was used to detect expression of phospho-FoxO3 (S253) in response to U0126 treatment in MDA-MB-231 human breast cancer cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody detected the expression of phospho-FoxO3 at S253 site in MDA-MB-231 cells.

Additional Notes

None

Related Categories

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Summary

The Good

Detects specific band with clean background

The Bad

Signal is weak

The Bottom Line

This antibody worked to detect the expression of phospho-FoxO3 at S253 site, although the signal is weak.

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