Thermo Fisher (Applied Biosystems)
Oncostatin M primer/probe set
Hs00968300_g1
We research chronic sinus disease, and because we work on human samples it is important that we have reliable assays that do not require repeating to ensure accurate results. The oncostatin m primer probe set from Applied Biosystems is accurate and easy to use.
RT-PCR
RNA from human sinus tissue lysates
This primer/probe set is a 20x solution, so if your PCR reaction is 20uL, then it is only 1 uL/reaction.
Applied Biosystems offers a XS 75uL size so you can try it for under $100
Results using this primer probe were always reliable.
Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Sep;136(3):737-746.e4. doi: 10.1016/j.jaci.2015.01.043.
Super easy and reliable
More expensive than sequencing primers and probes
If you are doing RT-PCR for Oncostatin M use this primer/probe set.