Cell Signaling Technology
CBP (D6C5) Rabbit mAb #7389
7389
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We were screening CBP knockout candidates in mouse ES cells. We chose the antibody because the band on the image of Western blot on the antibody site looked specific and of the right size.
Western Blot
Whole cell lysates from mouse ES cells
1:1000 dilution overnight in 5% bsa buffer at 4C
5% bsa
1:3000 antirabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from BioRad
Knockouts showed no bands which is good. When bands showed up, there were multiple, however.
None
Somewhat specific
Multiple bands