Fig 1: Expression of CD93 in human cells and its response to LPS. (A) The level of CD93 transcription was evaluated by qRT-PCR and presented as the relative value after normalization to GAPDH. The transcription level of sCD93 in THP-1 cells was set as 1 and compared between different types of cells (n = 4 per group). (B) The level of CD93 translation was immunoblotted with GAPDH as a loading control and (C) the value of CD93 relative to GAPDH was presented with the value of THP-1 as 1 (n = 4 per group). (D) HUVECs were harvested at the designated time point after LPS treatment and immunoblotted for CD93 expression. β-actin was used as a loading control. (E) The quantification of (D) was presented with the expression level at baseline set as 1 (n = 4 per group). (F) The localization of CD93 in the HUVECs was visualized by immunofluorescent staining with CD93, CD31, and DAPI after 48 h of 500 ng/mL LPS stimulation. Scale bar, 50 µm. (G) The supernatant of cultured HUVECs was harvested after 8 h or 24 h of 500 ng/mL LPS stimulation and the sCD93 level was measured by ELISA. All values are fold change compared to the value of 8 h without LPS stimulation as the control. The difference was compared between the PBS and the LPS group (n = 4 per group). Data are presented as the mean ± SE. * p < 0.05.