Life Technologies
ArC™ Amine Reactive Compensation Bead Kit
A-10346
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In contrast to compensating for fluorophore antibodies, to compensate for the live/dead stains, amine linked beads are required. This further allows differentiation of live or dead cells from staining as opposed to the cell's morphology.
Flow Cytometry
ArC™ Amine Reactive Compensation Beads
These beads are only used for one well, to compensate for the live/dead fixable stain. In a round bottomed well, 2 drops of the positive and 2 drops of the negative beads are added. These are then spun down and resuspended in 50ul of MACS buffer, before 0.5ul of live/dead stain is added. Incubate these beads for at least 15 mins at 4C in the dark, before washing the beads twice with MACS buffer. Fix the beads in 2% formaldehyde prior to running on the Flow Machine.
These beads are only for amine-linked dyes, such as the live dead stain, not for individual antibody conjugates.
These beads successfully allow the differentiation between live/dead stained beads (cells) or not.
None
Ease, speed and functionality
Easy, efficient beads for the compensation of live/dead staining prior to flow cytometry acquisition
Product Name: ArC Amine Reactive Compensation Bead KitSupplier Page