IL-10 Antibody for Intracellular Detection in Humans

January 14, 2016

Internal Medicine
University of California
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Company:

BioLegend

Product Name:

PE anti-human IL-10 Antibody

Catalog Number:

506804

Human IL-10 producing cells were detected in PBMCs stimulated under various conditions from arthritis patients.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Peripheral blood mononuclear cells

Primary Incubation

10ul per million cells for an hr

Blocking Agent

Human AB serum in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

PE color on flow cytometer

Results Summary

While positive cells can be detected, isotype has tendency to bind as well, therefore careful gating is very much required and dilution and blocking of the sample is extremely critical to the assay that one performs.

Additional Notes

Isotype is a must

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Summary

The Good

Detects positive cells, color intensity is good

The Bad

Isotype binds, difficult to conclude positive population at times

The Bottom Line

If carefully gated and antibody used in proper dilution after blocking, positive cells can be detected. The company needs to adjust the isotype specificity.

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