KPL
Anti-Mouse IgG (H+L) Antibody, Human Serum Adsorbed and Peroxidase Labeled
074-1806
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We used this antibody to detect mouse primary antibodies in Western blots. I used the antibody because of the low non-specific binding, which gave a low background signal.
Western Blot
Primary Human Epithelial Cells
Primary anti-mouse antibody, overnight, 4 degrees
5% Milk or 3% BSA in 0.1% TBS-T
HRP at 1:5000 for 1 hr at room temperature
NA
Chemilumenescence
The HRP secondary was used to detect an anti-actin mouse primary. The results show a single band at the correct molecular weight.
None
Good antibody to detect primary mouse antibodies
This is a good secondary antibody.