Very Good Total AMPK Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technologies

Product Name:

AMPKβ1/2 (57C12)

Catalog Number:

4150

I am investigating the role of the AMPK-Pathway after extracellular vesicle treatment. I chose this product since I am also using the phospho AMPL antibody from Cell Signaling and it is showing good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

RT, 1h, 5% milk in TBST

Secondary Incubation

RT, 1h, 5%mil in TBST, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad clarity

Results Summary

Very good antibody for the detection of AMPK.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Very good AMPK-Antibody

Share your experience with other scientists. Write a Review! »

Join the discussion