Omega Bio-Tek
E.Z.N.A. Gel Extraction Kit
D2501-01
Standard cloning and sub-cloning procedures will oftentimes depend upon digesting and purifying either vector or gene of interest DNA. There are many kits available to perform this purification. Most of these kits have anion exchange at the center of their protocols with each company developing a proprietary buffer and pH conditions. For cost savings and a straightforward protocol the Omega Bio-Tek Gel Extraction Kit delivers clean reproducible results at an affordable price.
DNA purification
Agarose bound DNA, restriction digests
The protcol is based off standard low melt gel protocols. Briefly, the sample is excised from the gel and weighed. A chaotropic salt buffer is then added at a specified ratio and the entire sample is heated to 60°C for 5-10min. The melted sample is then added to a spin column or onto the luer lock of a vacuum manifold. The sample is bound to the column, washed several times and then removed from the column with elution buffer.
Have the heat block equlibrated prior to start of the protocol. Add ethanol to wash buffer prior to starting.
DNA with a high purity is usable in downstream applications from sub cloning to PCR.
None
Quick, easy and inexpensive.
Plastic waste
An affordable kit for the purification of DNA from either agarose gel on enzymatic reactions.