Abcam
Anti-CDYL antibody
ab5188
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Browse Similar Products: CDYL Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use western blots and subsequent sequencing to screen potential knockout clones. We chose this antibody because it had good reviews with rat cell lysates and was chip grade.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk in PBST
1:3000 anti-rabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Results show a lot of bands--hard to tell which is specific. We confirmed one of these clones (#4) as a true knockout through sequencing, and the WB kind of confirms this since the staining is lighter than the other lanes. However, there is still the issue of multiple bands.
None
Works
Lots of nonspecific bands
It works, but isn't very specific. Other methods would be needed to confirm presence/absence of the protein.