R&D Systems
Mouse GLI‑2 Antibody
AF3635
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Browse Similar Products: Gli2 Western blot Antibodies
One of the goals of my project is to carry out protein-protein interactions with components that are relevant to the hedgehog signaling. A first step in the process is to test the antibodies to determine the expression in either over-expression or endogenous levels. The goal of this experiment was to test the expression levels of Gli2 protein in NIH3T3 cells.
Western Blot
NIH3T3 cells
1:200 dilution in blocking buffer for 16 hr at 4 deg
5% Milk in 1x TBS-T (0.1% Tween)
Donkey anti-goat IgG HRP secondary antibody for 1 hr at room temp
None
ECL pico for 5 min
The antibody detects a band at ~200 kDa (the expected size for Gli2). Based on my experience working with this antibody, it is a bit tricky working with this antibody. Sometimes you can detect the signal with ECL pico but other times, I need ECL Femto
Detects a band at ~200 kDa (the expected size for Gli2)
Can be tricky sometimes to detect a good signal
Works okay