Abcam
Anti-KAT6B / MORF antibody
ab191994
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Browse Similar Products: KAT6B Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use western blots and subsequent sequencing to screen potential knockout clones. We chose this antibody, because we've had some luck with abcam antibodies before.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk in PBST
1:3000 anti-rabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Could see almost no bands in the right kDa range. The only band, as pictured, is super faint and probably non-specific. Only two of these clones were confirmed as knockouts through sequencing, although you would never be able to tell which ones from the WB with this antibody.
None
Easy refund process for abcam
Antibody doesn't work
Would not recommend for mouse ES cells.