Good General Purpose B Cell Marker

January 20, 2016

Research Participation Program
Oak Ridge Institute for Science and Education
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse/human CD45R/B220

Catalog Number:

103232

We study different methods of activation and inhibition of B cells. We use a variety of anti-B220 clones and colors in our flow cytometry panels. This anti-B220 antibody gives consistent results, strongly labeling mouse splenic B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Magnetically purified mouse splenic B cells

Primary Incubation

1:100 dilution of Alexa Fluor 700 anti-mouse CD45R/B220 on ice for 10 minutes

Blocking Agent

1xPBS+2%FBS+2mM EDTA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR Fortessa

Results Summary

In a sample containing purified mouse splenic B cells, all of the cells stain strongly positive for surface B220 using this antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Reliable, strong labeling of splenic mouse B cells

The Bad

No drawbacks observed.

The Bottom Line

This is a reliable antibody that will strongly label mouse splenic B cells. It is a useful product to have on hand to give you flexibility when designing multi-color flow panels.

Share your experience with other scientists. Write a Review! »

Join the discussion