Anti-UPF1 in Western Blot

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

anti-UPF1

Catalog Number:

HPA019587

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BPH1 cells

Primary Incubation

1:1000 diluted in 1xTBST, incubation for 1h at room temperature

Blocking Agent

5% milk powder solution

Secondary Incubation

HRP-conjugated secondary antibody, 30 min incubation at room temperature

Tertiary Incubation

None

Detection

ECL detection method

Results Summary

Specific detection of UPF-1 protein in Western-Blot.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific antibody

The Bad

Nothing

The Bottom Line

Good antibody for the detection of UPF1.

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