Excellent Anti-Mouse CD45 Antibody

January 19, 2016

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

Violet Fluor450 Anti-Mouse CD45

Catalog Number:

75-0451

Browse Similar Products: CD45 Flow Cytometry Antibodies

Total mouse peritoneal cells were analyzed. To identify leukocytes and remove red blood cells, CD45 discrimination was used.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total Peritoneal Leukocytes

Primary Incubation

1:400 violetFlour450 CD45 - 30 minutes on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II

Results Summary

After gating away debris and doublets it is very easy to identify residual red blood cells using Tonbo's CD45.

Additional Notes

None

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Summary

The Good

Price and quality results

The Bad

None

The Bottom Line

As good as other CD45 reagents we have tested in the 450 detector off 405 laser at about half the price.

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