Alexa Fluor® 700 Mouse IgG2a, kappa Isotype Control

January 20, 2016

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 Mouse IgG2a, kappa; Isotype Ctrl

Catalog Number:

400247

This experiment involved detection of B cell/T cell lymphoma using flow cytometry. This antibody is used to determine the negative population, or cells that don't stain with other antibodies and hence works as a good negative control.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood in EDTA

Primary Incubation

1ul of the isotope for 30mins in the dark at 4 degrees.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Serves as a good isotype control. As seen in the image below, shows distinct negative population in the lower left plot.

Additional Notes

None

Image Gallery

Summary

The Good

Serves the purpose of an isotope control

The Bad

None

The Bottom Line

Good Antibody

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