Miniprep Kit

U of Miami
Biology
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

GeneJet Plasmid Miniprep Kit

Catalog Number:

K0502

This kit is used to purify plasmid from bacteria. The kit has simple instructions and produces high quality plasmids with good 260/280 and 260/230 readings. It is also a little cheaper than other kits. Overall, this is a good plasmid miniprep kit.

Experimental Design and Results Summary

Application

Plasmid Purification

Starting Material

None

Protocol Overview

Grow bacteria containing plasmid overnight. Spin down culture to pellet bacteria. Follow kit's instructions for resuspending, isolating, and purifying and eluting plasmid DNA.

Tips

Wait 1 minute before eluting to help get off all the DNA on the column

Results Summary

The plasmid DNA produced is high quality with good 260/230 and 260/280 numbers. The plasmid has also been used in subsequent cloning applications. Thus, it is good to use with downstream applications.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use

The Bad

Nothing is very wrong with the kit

The Bottom Line

This is a high quality kit for plasmid purification.

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