Santa Cruz Biotechnology
Cortactin Antibody (H-191)
sc-11408
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Human aortic smooth muscle cells were quiesced and time-course effect of chemokines was tested on cortactin expression.
Western Blot
Aortic Smooth Muscle Cell lysates
5% skim milk, 1 hr at room temperature
5% skim milk
1:1000 dilution/ over night / 4 deg C
Goat anti-rabbit, 1:4000, room temperature for 1 hr
Horseradish peroxidase (HRP)
Cortactin expression is constant with time-course treatment of chemokine in human aortic smooth muscle cells.
None
This antibody detects human, mouse and rat cortactin.
The antibody gives non-specific bands in human aortic smooth muscle cells
The antibody is good and detects cortactin band but gives non-specific bands.