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APC anti-human CD4 Antibody
344613
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I am evaluating the expression of CD4, CD5 and CD7 on Jurkat cells after CRISPR/Cas9 mediated knock-out as a proof-of-principle experiment. We were trying to establish whether the knockout works, and it was easiest to address that by looking at the surface receptors. This product was used for verification of CD4 expression on a Jurkat cell line.
Flow Cytometry
Jurkat cells
1:200, 30 min on ice
0.5% BSA, 4% FBS
NA
Fluorescence
Very good staining (Jurkat cells express high levels of CD4)
None
Simple to use
Very good product