IL-2 Receptor Antibody

January 14, 2016

Neuroscience
Wright State University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bosterbio

Product Name:

Anti-IL2RA antibody

Catalog Number:

PA1747

Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Jurkat Lysates

Primary Incubation

1:1000, 16hrs, 4 degrees

Blocking Agent

5% Milk

Secondary Incubation

1:10,000 Mouse anti-rabbit

Tertiary Incubation

None

Detection

ECL solution

Results Summary

Got one single band at the correct size.

Additional Notes

None

Related Categories

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Summary

The Good

Very sensitive, can use at low concentration

The Bad

Not much bad to be mentioned. Works everytime.

The Bottom Line

Great antibody. Works well with whole cell lystes from our cell lines.

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