Promega
Pure Yield Plasmid Mini Prep Kit
A1223
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Our lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very simple, requires much less time, and is easy to adopt for the trainee students. We found the quality of DNA to be as good as Qiagen’s mini prep kit.
Plasmid DNA isolation and transfection
Plasmid DNA
It is very important to avoid using more than 5 ml of culture for high copy plasmids. Using pre heated (55C) water yields a higher number of plasmids. Typically I used get 6-8ug of plasmid DNA (13kb size).
Avoid overgrown culture
Obtained high quality lenti viral plasmids for tranfection into mammalian cells.
None
Ease of use.
We strongly recommed this Promega Kit with lesser cost compared to other kits.
Product Name: PureYield Plasmid Miniprep SystemSupplier Page