The Antibody Stains CD14+ Small Intestinal Cells Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

FITC Rat Anti-Mouse CD14

Catalog Number:

561710

We wanted to examine the proportion of CD14+ cells in the small intestine in a diseased vs healthy state. To do so, we wanted to use flow cytometry and therefore needed an antibody to identify the CD14+ cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of small intestinal cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR II flow cytometer

Results Summary

The CD14+ cells in the small intestine were stained well with the antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Stains the CD14-expressing cells well

The Bad

None to mention

The Bottom Line

The antibody clearly distinguishes CD14+ cells in the small intestine.

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