Abcam
Anti-NQO1 [clone A180]
ab28947
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Browse Similar Products: Anti-NQO1 Western Blot Antibodies
We evaluated relative expression of NQO1 across a panel of ovarian cancer cell lines. I chose this antibody because I typically have success with Abcam antibodies by Western, and the price was reasonable.
Western Blot
Ovarian cancer cell line panel - lysates
1:2000 in 5% milk in TBS-T; incubated overnight at 4 degrees C on a rocker
5% milk in TBS-T
1:5000 anti-mouse HRP in 5% milk in TBS-T; incubated 30 minutes at room temp on a rocker
NA
HRP Chemiluminescence
There was high, unexplainable background on the blot. Even after the assay was repeated 3 times, there remained high background. The antibody was used initially at 1:1000, then at 1:2000 and 1:2500, but the background remained at 1:2000 and the bands were far less detectable at 1:2500.
None
A band of correct size was detected
There was high background on the blot
Due to the high background, I would not recommend using this antibody