Good Separation of CD4 Population

March 24, 2016

Pharmaceutical Technology
University of Basque Country
Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD4 (Domain 1)-PE, rat (clone: REA482)

Catalog Number:

130-107-668

We analyzed the CD4 and CD8 population in lymph nodes. We chose Miltenyi antibodies for this purpose and got good results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph nodes

Primary Incubation

1:100

Blocking Agent

Blocking reagent from Miltenyi

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry in PE channel

Results Summary

Good separation from unlabelled cells with 1:100 dilution and considering diluting even more.

Additional Notes

None

Image Gallery

Summary

The Good

Good price and quality

The Bad

None

The Bottom Line

I would recommend ordering this antibody

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