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F4/80 PE-cy7 conjugate against mice
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The peripheral lymph node cells were used to detect the presence of a specific class of F480+ macrophages.
Flow Cytometry
Peripheral lymph node cells
1:500 in Fc block in dark on ice
Fc block
NA
In PE-cy7 channel
If properly compensated the antibody does detect the macrophages very nicely.
None
Strong color, strong binding
Sometimes there is leakage especially when antibody is old
If avoiding PE channel or if compensating properly against PE this antibody is a good one to detect mouse macrophages.