Western Blot for Albumin

Environ. Med.
University of Rochester
Postdoc

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Quality of Results

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Company:

Sanra Cruz Biotechnology

Product Name:

ALB (F-8) Antibody

Catalog Number:

sc-374670

We performed isolation of plasma derived exosomes and determined the levels of albumin in isolated exosomal proteins.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Exosomal lysates from human plasma

Primary Incubation

1:1000 in 5% milk at 4 degrees overnight

Blocking Agent

5% Milk

Secondary Incubation

1:10,000 Goat Anti-Rabbit IgG, (H+L), HRP conjugate

Tertiary Incubation

NA

Detection

ECL

Results Summary

We were able to detect variable levels of plasma derived albumin in exosome lysates.

Additional Notes

None

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Summary

The Good

Works for both human and mouse samples

The Bad

Noted none

The Bottom Line

We were able to detect variable levels of plasma derived albumin in exosome lysates

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