Detection of Dendritic Cells

Immunology
Universitätsklinikum Hamburg-Eppendorf
BTA

Overall

Quality of Results

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Company:

BioLegend

Product Name:

APC/Cy7 anti-human CD1c

Catalog Number:

331520

We use this antibody in a 15-colour panel on whole cord blood. Through CD1c human myeloid dendritic cells can be detected nicely.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole cord blood

Primary Incubation

0.1µL CD3 in 50µL FACS buffer added to 50µL blood for 30min at RT

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

Good detection of CD1c+ cells.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody gives very good results in low concentrations.

The Bad

None

The Bottom Line

This antibody is good for multi-color panel.

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