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PE anti-mouse TNF-α Antibody
506306
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.
Flow Cytometry
Murine Lymphocytes
1 hour on ice with primary antibodies
Blocking with 2% BSA and Fc block (1:100)
1:50 dilution on ice with intracellular staining antibodies
None
FACS
Detected TNFa expression in lung lymphocytes on CD4+ population in response to mycobacterial antigens.
Good flow cytometry antibody for TNFa intracellular staining
Good flow cytometry antibody with minimal cross reactivity