Illumina
Nextera XT DNA Library Preparation Kit
FC-131-1024
We routinely do bacterial isolates genome sequencing and for that we use Nextera XT DNA library prep kit. Usually we get very good quality library at 50 to 100 nM concentration which are then equally pooled with indexed libraries and finally 20 pM was loaded onto Miseq. Over 90 percent of reads had Q score more than 30.
Bacterial genome sequencing
E. coli genomic DNA
Bacterial isolates were used for genomic DNA preparation. Then we use Nextera Index Kit to introduce indexing (multiplexing the isolates). Library preparation is very simple and just follow standard protocol guide form the kit. Finally we load 20 pM denatured libraries onto Miseq platform for sequencing.
None
All the libraries prepared were of good quality as checked by Agilent bioanalyser using DNA chip. Over 90 percent of reads pass the filter and had a Q score greater than 30.
Very easy to perform protocol. Entire protocol can be finished in less than 3 hrs with hands-on-time less than 30 minutes.
Highly recommended for large scale genome sequencing different bacterial isolates.