Human CD3 (Brilliant Violet 650™)

March 23, 2016

Immunology
Universitätsklinikum Hamburg-Eppendorf
BTA

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-human CD3

Catalog Number:

317324

We are staining this antibody on whole cord blood in a 15-clolour panel. It works very well to distinguish the T cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole cord blood

Primary Incubation

0.3µL CD3 in 50µL FACS buffer added to 50µL blood for 30min at RT

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

Nice detection of human CD3+ Tcells because the positive and negative population can be separated easily.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody gives very good results in low concentrations.

The Bad

None

The Bottom Line

This antibody is good for multi-colour panel.

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