CD34 Antibody

Pathobiology
University of Guelph
PI

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Quality of Results

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Company:

Biorbyt

Product Name:

CD34 antibody

Catalog Number:

orb248261

This mAB is supposed to react with human, rat, cow, monkey and dog 34 in formalin-fixed tissues. It was tested against dog and horse bone marrow sections and there was no staining.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Bone marrow sections

Primary Incubation

1/100 and 1/400 dilutions, antigen retrieval with heat and proteinase K at pH 6 and 9

Blocking Agent

Universal block

Secondary Incubation

Synthetic polymer, 30 min

Tertiary Incubation

NA

Detection

Nova red chromogen

Results Summary

No staining whatsoever.

Additional Notes

None

Related Categories

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Summary

The Good

Looked promising

The Bad

Did not work

The Bottom Line

Did not work on canine or equine tissue.

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