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Solid Dye From Cytek Biosciences for Live/Dead Cell Staining
(1)
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Tonbo Biosciences
October 23, 2023
This live/dead stain delivers solid results on mouse and human LPMCs. Optimal staining result at 1:1000.
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Leak Proof Cell Culture Flask From Greiner
(1)
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Greiner Bio-One GmbH
October 23, 2023
Great for cell culture. The holes provide gas exchange without allowing contamination. Has a good seal and the tissue culture bottom allows ...
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Vacuum Driven Filtration System From Millipore is Great for Filtering Small Cells
(1)
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MilliporeSigma
October 23, 2023
We use this for filtering small amounts of reagent or cell media. Works quickly and easily and helps prevent contamination.
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Bright and Specific Anti-CD103 Antibody From BioLegend for Flow Cytometry
(1)
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BioLegend
October 23, 2023
The PEdazzle594 CD103 antibody can be used to identify tissue resident CD8+ T cells within immune cell populations isolated from mouse lungs...
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Multi Use PCR System From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 23, 2023
The StepOnePlus Real-Time PCR System is a highly versatile platform that excels in accommodating a wide range of PCR applications. Its ...
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Decent Antibody From Thermo Fisher but, Not the Best
(1)
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Thermo Fisher Scientific
October 20, 2023
When analyzed by flow cytometry, there is some variability in the staining, which produces a smear. Decent, but not the best.
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Good Anti-KLRG1 Antibody From BioLegend for the Flow Cytometric Detection of Effector T Cells
(1)
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BioLegend
October 20, 2023
The BV 711 KLRG1 antibody can be used for identifying effector CD8+ T cells within immune cell populations isolated from mouse lungs, spleen...
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Foolproof Nuclear Stain From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 20, 2023
We do immunofluorescence experiments to visualize cells seeded on chips. This nuclear stain has never failed us in visualizing cell nuclei.
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Puromycin From Thermo Fisher is Ideal for Antibiotic Selection
(1)
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Thermo Fisher Scientific
October 20, 2023
Puromycin (puromycine) is an aminoglycoside antibiotic originating from Streptomyces alboniger. It acts as an aminoacyl tRNA analog and ...
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Cdkn1a Probe From ACD Bio for RNA-ISH in Rat Samples
(1)
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ACD Systems International Inc.
October 20, 2023
We used p21 probe (Rn-Cdkn1a) to perform RNA-ISH in rat samples. Probe with ref. 42851 from ACD is great for detecting mRNA levels of cdkn1a...
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One of the Best Nuclease Free Waters From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 20, 2023
The aim of the study was real-time quantification of a gene of interest in human PBMCs. The qRT-PCR reagent was prepared with nuclease-free ...
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Great Kit From Qiagen for Bacterial Plasmid DNA Extraction
(1)
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October 20, 2023
We use this kit daily in my lab. Simple and fast, it yields high-quality DNA for any downstream applications. After the transformation of ...
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A Great IBA1 Antibody From Fujifilm for Immunohistochemistry
(1)
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October 19, 2023
I've used this antibody to study the localization and morphology of microglia/macrophages in a mouse retina. Immunostaining of a cross-...
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Reliable Human T3 ELISA Kit From Boster
(1)
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BosterBio
October 19, 2023
This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits.
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High Quality Rheometer From Anton Paar for Measuring Pluronics
(1)
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October 19, 2023
The Modular Compact Rheometer MCR302e is an exceptional rheometer with versatile capabilities, making it an ideal instrument for measuring ...
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Rapid Glutamine Quantification Kit From BioVision
(1)
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October 19, 2023
I have used this glutamine colorimetric assay kit to understand the metabolic reprogramming in virus-infected primary human epithelial cells...
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Filter Plate From Millipore Suitable for ELISPOT or MTS Assays
(1)
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MilliporeSigma
October 19, 2023
We use these plates to coat INF-y antibodies for ELISPOT or for cytotoxic assays. It is sturdy with deep wells for easy multi-channel ...
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Great Kit for Live/Dead Flow Calibration
(1)
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Thermo Fisher Scientific
October 18, 2023
We use these beads for live/dead compensation tubes. Works well with zombie green dye. We use one drop of reactive beads first, then add the...
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Excellent Dye From Invitrogen for Nuclear Counterstain (DAPI)
(1)
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Thermo Fisher Scientific
October 18, 2023
DAPI is a water-soluble dye and emits blue fluorescence upon binding to AT regions of DNA which makes it a popular nuclear counterstain. ...
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Superb Reagent to Re-stimulate Human T-Cells
(1)
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Miltenyi Biotec
October 18, 2023
I used cytoStim reagent as a positive control to restimulate CD4+ and CD8+ T-cells present in PBMCs. After stimulation for 4-8h PBMCs were ...
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Cdkn2a Probe From ACD for the Accurate Detection of mRNA Levels
(1)
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October 18, 2023
We used p16 probe (Rn-Cdkn2a) to perform RNA-ISH in rat samples. Probe with ref. 423861 from ACD is great for detecting mRNA levels of ...
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Extraordinary Antibiotic From Thermo Fisher
(1)
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October 18, 2023
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein ...
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Reagent From Gibco Works Very Well to Dislodge Cells While Being Gentler Than Trypsin
(1)
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Thermo Fisher Scientific
October 17, 2023
Works very well to dislodge cells while being gentler than trypsin so we can still perform flow cytometry on them
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Satisfactory PCR Tubes From Applied Biosystems™
(1)
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October 17, 2023
MicroAmp Fast Reaction Tubes are highly durable and chemically resistant, making them ideal for PCR applications, as they can withstand ...
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Decent Antibody From IBL for the Detection of IgAN
(1)
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IBL- America
October 17, 2023
Our group is developing personalized antibody-based treatments for IgA nephropathy. As such, we need specific markers for IgAN, to measure ...
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Superb Kit From BioLegend for Live/Dead Cell Analysis
(1)
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BioLegend
October 17, 2023
Does a great job staining dead cells for accurate live/dead analysis in flow cytometry. Be sure to keep it in the dark, as it is light-...
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DHEA Competitive ELISA Kit From Boster
(1)
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BosterBio
October 17, 2023
We used this kit to analyze saliva DHEA levels, with great success level. Validation of this assay has been straightforward. The assay ...
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Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well
(1)
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BosterBio
October 17, 2023
The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood...
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Antibody From StressMarq for Detecting P. falciparum HSP70 in Clinical Samples is Up to Standard
(1)
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StressMarq Biosciences Inc
October 17, 2023
We aimed to determine the level of pfHSP70, one of the Plasmodium falciparum chaperone proteins, in the peripheral blood of malaria patients...
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Great Anti-Human CD45 Antibody From BioLegend is Easy to Work With
(1)
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BioLegend
October 16, 2023
We are a cancer immunotherapy group, using cell-based approaches. Ultimately our products are tested in animal models, so good markers are ...
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Very Good CD64 Antibody From BioLegend
(1)
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BioLegend
October 16, 2023
Great CD64 antibody to use when staining intestine immune cells. Clear positive population, although the signal could be a bit brighter.
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Decent Anti-CD127 Antibody From BioLegend for the Flow Cytometric Detection of Memory T Cells
(1)
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BioLegend
October 16, 2023
The BV 421 CD127 antibody can be used for identifying memory CD8+ T cells within immune cell populations isolated from mouse lungs, spleen, ...
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Excellent Kit From Thermo Fisher for Visualizing Proliferating Mouse Cells at Any Stage
(1)
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Thermo Fisher Scientific
October 16, 2023
I used this kit to assess the cell proliferation status within the mouse's inner ear. This kit was very useful and worked well with no ...
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Excellent MUC18 Antibody From Abcam for Endothelial Cells
(1)
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Abcam
October 16, 2023
We needed to visualize cultured endothelial cells. MUC18 provides a great fluorescence for these cells.
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Great Reagent From Gibco Highly Recommended for Counting With No Contamination
(1)
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Thermo Fisher Scientific
October 16, 2023
I use this for sterile cell culture and counting. It doesn't harm the cells and never contaminates our culture. It also doesn't interfere ...
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Isolectin B4 From Vector Laboratories to Easily Stain the Endothelium
(1)
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October 13, 2023
Isolectin B4 was used to stain endothelial cells in heart samples obtained from rats. Staining is rapid, easy, and (almost) always works ...
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Anti-Human CD8 Antibody From BioLegend Works as Expected Giving High Quality Results
(1)
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BioLegend
October 13, 2023
I used this antibody to determine the percentage of CD8+ T cells in my expanded T cell population. This antibody generated a good signal ...
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Efficient High-fidelity DNA Polymerase From Invitrogen for Site-Directed Mutagenesis
(1)
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Thermo Fisher Scientific
October 13, 2023
We study how certain mutations affect viral replication. We construct our mutant cDNA infectious clones by PCR and this DNA polymerase mx is...
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CD14 MicroBeads From Miltenyi is an Easy and Reliable Way to Separate PBMCs
(1)
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Miltenyi Biotec
October 13, 2023
We use anti-human CD14 microbeads to purify monocytes and macrophages from human peripheral blood mononuclear cells (PBMC), as CD14 antigen ...
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Good Human IL-6 ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
Interleukin 6 (IL-6) is an inflammatory cytokine. I used a human IL-6 ELISA kit to detect levels of IL-6 in cell supernatant after 24 hours ...
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Highly Recommended Antibody From Cell Signaling for Western Blot
(1)
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Cell Signaling Technology
October 12, 2023
Clean blots, sensitivity is good, great for blotting for tubulin as a loading control. They are used in MCF10a cells.
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Reliable Human IFN-gamma ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
IFN-gamma are known antivirals and are produced by immune cells in defense when one is exposed to the virus. I have used a Human IFN-gamma ...
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Excellent Reagent Mix From New England Labs for the Assembly of Large DNA Plasmids
(1)
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New England Biolabs
October 12, 2023
We study how different resistance mutations can affect Dengue virus replication. To do that, we need to obtain viral genetic material ...
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Great Cell Culture Reagent From Genesee
(1)
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Genesee Scientific
October 12, 2023
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing ...
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L-Glutamine From Thermo Fisher is Ideal to Culture HL-1 Cells
(1)
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Thermo Fisher Scientific
October 12, 2023
L-glutamine is diluted to 2 mM in Claycomb medium with 10% FBS, 1 % penicillin/streptomycin, and 0.1 mM noradrenaline and used for the ...
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Collagenase Type VIII From Sigma Aldrich for Tissue Digestion
(1)
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MilliporeSigma
October 12, 2023
My research area includes studying the role of inflammation in the development of colorectal cancer. I often use different types of ...
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Excellent Peroxidase-Labeled Antibody From Seracare for Focus Forming Assay
(1)
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October 11, 2023
I used this antibody as a secondary antibody in the focus-forming assay to determine the dengue virus titer and obtained satisfactory ...
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Decent CD19 Antibody From BD Biosciences
(1)
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October 11, 2023
When used for flow cytometry on intestinal cells, there will be some background, but the B cell population is clear.
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CellStar's 6-Well Cell Culture Plate is Ideal for Tissue Culture
(1)
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Greiner Bio-One GmbH
October 11, 2023
We put 1E6 dendritic cells per well which are adherent and grow very well and uniformly on the TC bottom. The plate is sturdy and never ...
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Straightforward, High Quality Anti-CD4 Antibody From BioLegend
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BioLegend
October 11, 2023
I used this antibody to determine what percentage of my T cell product was CD4+. I was able to quantify the subpopulation with no issues.
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