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Zombie Aqua Viability Dye
77143
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We are a B cell vaccine lab. In some of our FACS panels, we use Zombie Aqua viability dye to gate on live cells.
Flow cytometry
Human and mouse splenocytes, B cells
Follow manufacturer's protocol, and make sure to wash cells with PBS to get rid of FBS from cell suspension before staining.
Staining with Zombie aqua viability dye helps us differentiate between live and dead cells. Gating on live cells is important prior to analyzing the expression of surface and intracellular markers. The staining result remains the same on cells fixed using PFA/ FoxP3 Fix-Perm buffer kit.
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Easy to use, and good separation between live and dead populations in FACS analysis.
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Good viability dye.