Decent CD19 Antibody From BD Biosciences

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BV711 Mouse Anti-Human CD19

Catalog Number:

563036

Clone Number:

SJ25C1

Info:Supplier Page 

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A good antibody that can work very well. There can be some difficulty with compensation, but overall the antibody is okay.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse intestines stained with CD19-BV711 show a small population of clear positive cells that are also SSCA low as well as a large population of SSCA high cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining for B cells.

The Bad

Some background is present.

The Bottom Line

The staining is clear and the signal is strong. There can be some issues with compensation, but the antibody is ok to use.

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