The Human XL Cytokine Luminex Performance Panel Premixed Kit is a Simpler Alternative to Performing Multiple ELISAs

November 30, 2023

BlueskyReddit
George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D

Product Name:

Human XL Cytokine Luminex® Performance Panel Premixed Kit

Catalog Number:

FCSTIM18B- 09

In this study, we treated U87 glioblastoma cells with different cell treatment methods to determine the effect of each treatment on U87 cytokine secretion.

Experimental Design and Results Summary

Application

Measuring cytokine secretion

Starting Material

Cell culture supernatants

Tips

Ensure that machine is serviced before use and Cal/Ver processes have been performed, as poor fluidics will impact the results.

Results Summary

This product helped us determine the impact of each cell treatment on pro- and anti-inflammatory cytokine secretion, including dose-dependent responses.

DOI or PMID #

N/A

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Summary

The Good

Easy-to-perform workflow.

The Bad

Long workflow (over 8 hours) and complex software.

The Bottom Line

This is a great (and ultimately cost-effective) alternative to performing multiple ELISAs in a discovery phase of an experiment. However, the long workflow and difficulties with the machine (including low bead counts for several samples), combined with the high up-front cost may not be worth it for research/non-clinical use.

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