Bright and Specific Granzyme B Antibody From BioLegend

December 08, 2023

BlueskyReddit
NA
Charite Medical University Berlin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-human/mouse Granzyme B Recombinant Antibody

Catalog Number:

372213

Clone Number:

QA16A02

Human PBMC were stained with CD3/CD4/CD8, fixed, permeabilized, and then stained with Granzyme B. FIxation and permeabilization using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set. Antibody was diluted in 1X permeabilization buffer.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

PBMC

Primary Incubation

1:400 for 30 mins at RT

Blocking Agent

Human TruStain FcX™ (Fc Receptor Blocking Solution)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa X20

Results Summary

Great Granzyme B staining in PBMC but, not monocyte. 1:400 is an ideal concentration to separate positive and negative population. I think one could titrate further. But, it is not essential.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright and specific staining.

The Bad

N/A.

The Bottom Line

Bright and specific, highly recommended.

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