Antibody From BioLegend Works as Intended With Bright Signal

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

PE anti-mouse CD45.1 Antibody

Catalog Number:

110708

Clone Number:

A20

I needed to identify the T cells that were adoptively transferred from a mouse with a CD45.1 background to a wild-type C57BL/6 mice. Using this antibody, I was able to discriminate by flow cytometry the transplanted T cells by CD45.1 expression.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Primary mouse cells

Primary Incubation

30 minutes at 4C in the dark

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I was able to identify the percentage of CD45.1 cells from the tumor cells I had collected from a mouse that had received adoptively transferred T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Accessible in several fluorophores.

The Bad

As far as I can find, there's only one clone (A20).

The Bottom Line

This antibody works as intended and provides a clear and distinguishable signal.

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