Excellent Reagent From AmericalBio to Prepare Blocking Buffer for ELISA

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

americanBIO

Product Name:

Non-Fat Dry Milk OmniBlok

Catalog Number:

AB10109-01000

To detect antibodies against human common cold viruses in pre-pandemic serum samples, I have used non-fat dry milk from americanBio to prepare the blocking buffer for the ELISA.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human Serum/Plasma samples

Tips

Dissolve the powder in TBST buffer with gentle stirring.

Results Summary

Using this reagent to prepare the blocking buffer reduced the background absorbance and able to detect true antibody positive samples.

DOI or PMID #

N/A

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Summary

The Good

Reduces the background absorbance/ non-specific binding significantly.

The Bad

None.

The Bottom Line

Always prefer to prepare blocking buffer using nonfat dry milk Omni Blok.

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