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Good Human IL-6 ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
Interleukin 6 (IL-6) is an inflammatory cytokine. I used a human IL-6 ELISA kit to detect levels of IL-6 in cell supernatant after 24 hours ...
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Excellent Reagent Mix From New England Labs for the Assembly of Large DNA Plasmids
(1)
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New England Biolabs
October 12, 2023
We study how different resistance mutations can affect Dengue virus replication. To do that, we need to obtain viral genetic material ...
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Highly Recommended Antibody From Cell Signaling for Western Blot
(1)
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Cell Signaling Technology
October 12, 2023
Clean blots, sensitivity is good, great for blotting for tubulin as a loading control. They are used in MCF10a cells.
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Great Cell Culture Reagent From Genesee
(1)
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Genesee Scientific
October 12, 2023
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing ...
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Reliable Human IFN-gamma ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
IFN-gamma are known antivirals and are produced by immune cells in defense when one is exposed to the virus. I have used a Human IFN-gamma ...
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Collagenase Type VIII From Sigma Aldrich for Tissue Digestion
(1)
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MilliporeSigma
October 12, 2023
My research area includes studying the role of inflammation in the development of colorectal cancer. I often use different types of ...
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L-Glutamine From Thermo Fisher is Ideal to Culture HL-1 Cells
(1)
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Thermo Fisher Scientific
October 12, 2023
L-glutamine is diluted to 2 mM in Claycomb medium with 10% FBS, 1 % penicillin/streptomycin, and 0.1 mM noradrenaline and used for the ...
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Decent CD19 Antibody From BD Biosciences
(1)
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October 11, 2023
When used for flow cytometry on intestinal cells, there will be some background, but the B cell population is clear.
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A Good PAX6 Antibody From ThermoFisher Scientific
(1)
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Thermo Fisher Scientific
October 11, 2023
Our research focuses on understanding the role played by retina cells in neurodegenerative diseases. The specific goal of this experiment ...
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CellStar's 6-Well Cell Culture Plate is Ideal for Tissue Culture
(1)
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Greiner Bio-One GmbH
October 11, 2023
We put 1E6 dendritic cells per well which are adherent and grow very well and uniformly on the TC bottom. The plate is sturdy and never ...
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Straightforward, High Quality Anti-CD4 Antibody From BioLegend
(1)
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BioLegend
October 11, 2023
I used this antibody to determine what percentage of my T cell product was CD4+. I was able to quantify the subpopulation with no issues.
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Excellent Peroxidase-Labeled Antibody From Seracare for Focus Forming Assay
(1)
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October 11, 2023
I used this antibody as a secondary antibody in the focus-forming assay to determine the dengue virus titer and obtained satisfactory ...
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Great Cell Counting Slides From Logos Biosystems
(1)
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Logos Biosystems
October 11, 2023
Great for use in Luna Cell counter. We put 10uL samples in the end (9uL culture sample and 1uL stain). The slide has two ends so can be used...
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Great Anti-Albumin Antibody From Abcam for Rat Samples
(1)
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Abcam
October 10, 2023
Although Abcam does not indicate reactivity with rat samples, we conducted IHC-IF in the brain, and it worked perfectly well. Antigen ...
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Antibody From Invitrogen With Clear Signal But Was Average With My Cell Line
(1)
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Thermo Fisher Scientific
October 10, 2023
I used this antibody to attempt to determine B7H3 expression in a mouse tumor cell line and did not see any signal. Unfortunately, this ...
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Excellent Secondary Antibody From Invitrogen for IF
(1)
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Thermo Fisher Scientific
October 10, 2023
Goat anti-rabbit secondary antibody tagged with Alexa Fluor 488 is tested for immunofluorescence. Used at a concentration of 2.5 ug/ml for ...
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Good Protein Extraction Kit From Sigma Aldrich Requiring Protocol Modifications
(1)
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MilliporeSigma
October 10, 2023
The ProteoExtract Native Membrane Protein Extraction Kit is a valuable resource for membrane protein isolation; however, some protocol ...
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A Sturdy Flask From VWR With No Leaks Guaranteed
(1)
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VWR Scientific Products
October 10, 2023
This flask is great for cell culture. The holes on the lid provide gas exchange without allowing contamination. Has a good seal, and the ...
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Lamin B1 From Abcam for Rat Samples
(1)
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Abcam
October 10, 2023
We aimed to detect lamin B1 using rat brain samples, through IHC-IF. Overall, this antibody works fine.
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Mycoplasma Detection Kit From Lonza is Very Efficient
(1)
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Lonza
October 10, 2023
The MycoAlert® Mycoplasma Detection Kit is a vital tool for safeguarding the integrity of cell cultures, providing a user-friendly and ...
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Easily Accessible and Cheap TCR beta Antibody From BioLegend for Flow Cytometry
(1)
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BioLegend
October 09, 2023
The PerCPCy5.5 TCRbeta antibody can be used for identifying T cells within immune cell populations isolated from mouse lungs, spleen, and ...
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Great Reagent From Invitrogen for Flow Calibration
(1)
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Thermo Fisher Scientific
October 09, 2023
Provides great positive and negative populations for calibration before performing flow. Shows a great signal.
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Excellent Media From Corning for Cell Culture
(1)
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Corning Life Sciences
October 09, 2023
This media was used to culture cell amnion epithelial cells (AEC) in a study that investigated the induction of EMT in AECs in response to ...
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Cell Counter From Nexcelom Works Great!
(1)
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Revvity
October 09, 2023
The Spectrum Image Cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned ...
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Good UV Excitable CD4 Antibody From BD Biosciences for CD4+ T Cell Identification
(1)
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October 09, 2023
The BUV496 CD4 antibody works well for distinguishing CD4+ T cells within immune cell populations isolated from mouse lungs, spleen, and ...
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Programmable ELISA Plate Washer From TriContinent
(1)
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October 09, 2023
We are doing routine ELISAs daily. This multi-washer allows for a more convenient routine, doing three wash cycles with ease along with ...
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Decent Cleaved Caspase-3 Antibody From Cell Signaling for Western Blot
(1)
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Cell Signaling Technology
October 09, 2023
I used this antibody several times for the detection of apoptosis induction in whole-cell lysates of prostate carcinoma cells. The detection...
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Convenient PCR Master Mix From New England Biolabs
(1)
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New England Biolabs
October 06, 2023
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, ...
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Great Reagent From Biolegend for Flow Blocking
(1)
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BioLegend
October 06, 2023
We use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not ...
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Dependable cDNA Synthesis Kit From Bio-Rad
(1)
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Bio-Rad
October 06, 2023
I have used an iScript cDNA synthesis kit to study the differential gene expression after viral infection in primary human epithelial cells....
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Good Kit From New England Biolabs Ideal for the Isolation of Genomic DNA
(1)
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New England Biolabs
October 06, 2023
I extracted the genomic DNA from bacterial liquid culture with this kit to clone bacterial genes. This method yielded enough amount of ...
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Good Staining for MHC II With Antibody From BioLegend
(1)
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BioLegend
October 06, 2023
The goal of this research was to look for MHC presentation (in this case HLA DR, DP, DQ) after an activation-inducing treatment of CD14+ ...
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Fetal Bovine Serum From Cytiva is Ideal for Cell Culture
(1)
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Cytiva Life Sciences
October 06, 2023
We use FBS in many media, including our freeze media, T cell media, and FACs buffer. This FBS works well to keep cells growing and alive. We...
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Highly Recommended Criterion TGX Stain-Free Precast Gel From Bio-Rad
(1)
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Bio-Rad
October 06, 2023
This is a read-to-use gel that I used for Western blot and photoaffinity labeling experiments with bacterial lysate.
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Great Gr-1 Flow Cytometry Antibody From BioLegend for the Identification of Neutrophils
(1)
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BioLegend
October 05, 2023
The Spark blue 550 Gr-1 (Ly6-C / Ly6-G) antibody works well for identifying neutrophils from immune cell populations isolated from mouse ...
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ArC Beads From Invitrogen for Single Color Compensation Control for Fixable Amine Reactive Live & Dead Dyes
(1)
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Thermo Fisher Scientific
October 05, 2023
We are a B cell vaccine lab. For all our flow cytometry analyses of B cells, we use Arc amine reactive beads from Invitrogen for quick ...
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Amicon Tube From Millipore for Exosome Isolation
(1)
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MilliporeSigma
October 05, 2023
Currently working with Neutrophil Extracellular Traps (NETs) stimulated by exosomes derived from amnion epithelial cells under oxidative ...
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Reliable Protein Estimation Reagent From Bio-Rad
(1)
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Bio-Rad
October 05, 2023
I routinely use this Bradford reagent from Bio-Rad for the estimation of protein quantity in my samples (cell lysates, cell supernatant, and...
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Ideal Cell Freezing Container From Millipore
(1)
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MilliporeSigma
October 05, 2023
This device provides efficient freezing conditions for cell storage, it is a cooling container that gradually decreases temperature at a 1 °...
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Very Bright Marker for MHC I From BioLegend
(1)
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BioLegend
October 05, 2023
The goal of my research was to find increases in MHC I presentation on the cell surface of CD14+CD11c+ (Dendritic) cells when activated.
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Superb BCA Protein Assay Kit From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 05, 2023
This kit was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and ...
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Great Antibody From Cell Signalling for Western Blot
(1)
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Cell Signaling Technology
October 04, 2023
Very clean blot from MCF10a cells, and easy to optimise. Would definitely buy again to detect pMTORC2.
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Acridine Orange From Logos Stains Well
(1)
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Logos Biosystems
October 04, 2023
We used 2uL to stain the 18uL cell samples before putting them in the LUNA counter. The stain works well, though the cell counter will ...
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Bright Ly6C Antibody From BioLegend for the Identification of Monocytes and Neutrophils
(1)
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BioLegend
October 04, 2023
The BV711 Ly6C antibody works well for identifying monocytes / neutrophils from immune cell populations isolated from mouse lungs and ...
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24 well Tissue Culture Plate From Falcon Provides Uniform Cell Growth
(1)
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October 04, 2023
This plate works well with adherent dendritic cells and allows optimal and uniform growth. I also use this plate to separate DCs from T ...
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Antibody IP'd PANK4 From Bethyl Laboratories
(1)
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Bethyl Laboratories, Inc.
October 04, 2023
Antibody successfully IP'd PANK4 from MCF10a cells, but quite a messy blot and needs a lot of starting material.
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Reliable B7H3 Antibody From BD Biosciences With Low Levels of Background
(1)
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October 04, 2023
I used this antibody to determine the basal expression of B7H3 in my mouse tumor cell line. This antibody provided an excellent signal that ...
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Great Trypsin From Gibco
(1)
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October 04, 2023
I have been using it for years. I trust this brand for my media reagents. I would highly recommend it.
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TCRgd Antibody With Very Good Staining From Biolegend
(1)
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BioLegend
October 03, 2023
This TCRgd antibody gives a strong signal in mouse intestines and is great for flow cytometric analysis of gdT cells.
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Decent Multiplex Kit From Millipore Sigma to Determine Cytokine Levels
(1)
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Merck
October 03, 2023
We are aiming to characterize cytokine levels from a low volume of samples. This customizable kit allowed us to tailor six cytokines (from >...
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