Provides Satisfactory Staining for CD45.2+ Populations

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse CD45.2 Antibody

Catalog Number:

109836

Clone Number:

104

I utilized this antibody to detect what percentage of the cells I had isolated from my mouse tumors were CD45.2+. It worked as intended to provide a bright and distinguishable population that I could confidently say was CD45.2.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse primary cells

Primary Incubation

30 minutes at 4C in the dark

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I was able to identify a discrete population of CD45.2 cells in tumor I harvested from my mice, and allowed me to compare whether certain treatments increased lymphocyte infiltration into the tumor.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Clear and bright signal

The Bad

Pricey

The Bottom Line

I've already repurchased this since I went through it so quickly, but will be continuing to buy it so long as it produces quality signal.

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