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Brilliant Violet 421™ anti-human IFN-γ Antibody
506537
B27
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Functional characterization of T cell. Human PBMCs were stimulated with PMA/ionomycin for 5 hours in the presence of BFA. Cells were then surface stained with CD3/CD4/CD8, fixed, permeabilized, and then stained with IFNg. Fixation and permeabilization using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set. Antibody was diluted in 1X permeabilization buffer.
Flow cytometry
PBMC
1:200 for 30 mins at RT
Human TruStain FcX™ (Fc Receptor Blocking Solution)
N/A
Fortessa X20
Great IFNg staining in PMA-Ionomycin stimulated lymphocyte, but not monocyte. 1:200 could separate positive and negative population. However, would recommend titrated further (i.e. 1:600-800)
Bright and specific staining.
Highly recommended, specific and bright staining with good price.