Relatively Dim CD45.2 Antibody for Flow Cytometry

Results Summary

I used this product to identify CD45+ immune cells from cells isolated from the lung which contains a lot of epithelial and non-immune cell types. Cells were isolated from BALF using 1 ml of 1% BSA in PBS and from lungs through collagenase digestion; RBC lysis was performed using ACK buffer. Cells were then stained with viability dye followed by extracellular antibodies such as CD45, TCR beta, CD19, Ly6C, Gr1. I have not seen any spillover issues with this antibody and fluorophore combination.