Abcam
Anti-GAPDH antibody
ab8245
N/A
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The specific goal of this western blot experiment was to evaluate the expression level of protein of interest relative to GAPDH protein expression in cell lysate samples. This antibody worked for western blotting as a clear single band for GAPDH was observed.
Western Blot
Mouse fibroblast lysate
1:2000 at 4 degrees celsius for overnight
5% normal donkey serum
Secondary antibody at 1:1000 in 5% serum for 1 hour at room temperature
ECL reagent
A single robust band from mouse cell lysate samples. Very easy for optimization. I would definitely buy this antibody again. Just a little bit of this antibody needed as it has high sensitivity.
Very robust signal.
None.
Great signal as housekeeper protein. I would recommend this antibody for detecting GAPDH.